DOI: http://dx.doi.org/10.18203/2349-2902.isj20201878

Antibiotic susceptibility profiling in diabetic foot ulcer patients and evaluating treatment outcomes at a tertiary care hospital

T. J. Prasanna Kumar, Hari Babu Ramineni, Reshma Shaik, Suma Navya Yellavula, Virajitha Chandra

Abstract


Background: Diabetic foot infections are the predominant complications of diabetes mellitus with uncontrolled hyperglycemia levels. Multiple microbial invasion is the primary cause and the causative organism are aerobic gram positive cocci, gram negative bacilli such as Pseudomonas species, Escherichia coli, Klebsiella species, Proteus species and anaerobes.

Methods: This is a prospective, observational study conducted in the Department of General Surgery. 50 patients constituted the sample in our study. Patients pus and wound swab samples were collected using sterile and moist swab sticks under aseptic conditions and processed.

Results: More gram negative bacteria (66%) were isolated than gram positive bacteria (34%). E. coli (38%) is the major isolated microorganism. Among the isolated organisms many of them are susceptible to amikacin (60%), imipenem (52%) and ciprofloxacin (46%) respectively.

Conclusions: E. coli is the most common cause of infection. The antibiogram study showed an incidence of multiple resistant microorganisms to commonly used antibiotics.


Keywords


Diabetic foot ulcer, Gram negative and positive bacteria, Susceptibility antibiotics

Full Text:

PDF

References


Singer AJ, Tassiopoulos H, Kirsner RS. Evaluation and management of lower-extremity ulcers. N Engl J Med. 2018;378:302-3.

Shankar EM, Mohan V, Premalatha G, Srinivasan RS, Usha AR. Bacterial etiology of diabetic foot infections in South India. Eur J Intern Med. 2005;16:567-70.

Armstrong DG, Boulton AJM, Bus SA. Diabetic foot ulcers and their recurrence. N Engl J Med. 2017;376:2367-75.

Mutluoglu M, Uzun G, Turhan V, Gorenek L, Ay H, Lipsky BA. How reliable are cultures of specimens from superficial swabs compared with those of deep tissue in patients with diabetic foot ulcers?. J Diab Compl. 2012;26:225-9.

Banu A, Noorul Hassan MM, Rajkumar J. Prospective study of multidrug resistant bacteria causing diabetic foot ulcers in South India. J Sci. 2015;5:626-9.

Sharma VK, Khadka PB, Joshi A, Sharma R. Common pathogens isolated in diabetic foot infection. J Diab Compl. 2006;4:295-301.

Mehta VJ, Kikani KM, Mehta SJ. Microbiological profile of diabetic foot ulcers and its antibiotic susceptibility pattern. Int J Basic Clin Pharmacol. 2014;3(1):92-4.

Trojan R, Razdan L, Singh N. Antibiotic susceptibility patterns of bacterial isolates from pus samples in a tertiary care hospital. Int J Microbiol. 2016;7:89-93.

Bello OO, Edward O, Kelly BA, Mebude O, Bello TO. Antibiotic susceptibility profiles of bacteria from diabetic foot infections in selected teaching hospitals in South western Nigeria. Int J Microbiol. 2018;4:1-13.

Amini M. Determination of the resistance pattern of prevalent aerobic bacterial infections of diabetic foot ulcer. Iranian J Pathol. 2013;8:21-6.

Gangania PS, Singh VA. Bacteriological profile of diabetic foot infected patients. IJPAB. 2016;4:172-8.