Comparative study of impression smear with conventional mechanical corneal scrapping by potassium hydroxide (10%KOH) in diagnosis of fungal keratitis

Authors

  • Amit Patel Department of Ophthalmology, MRAMC, Ambedkarnagar, UP
  • P.D. Banswal Department of Ophthalmology, BRDMC, Gorakhpur, UP
  • A. Ghosh Department of Ophthalmology, MRAMC, Ambedkarnagar, UP
  • R.K. Jaiswal Department of Ophthalmology, BRDMC, Gorakhpur, UP
  • Shweta Goel Department of Ophthalmology, MRAMC, Ambedkarnagar, UP
  • M.P. Tandon Department of Ophthalmology, MRAMC, Ambedkarnagar, UP

DOI:

https://doi.org/10.18203/2349-2902.isj20162027

Keywords:

Impressing smear, Fungal keratitis

Abstract

Background:Diagnosis of fungal keratitis remains a challenge to an ophthalmologist due to variety of presentation of patients. If a prompt, aggressive and early treatment is not started disease turns indolent resulting in sloughing of cornea, serious loss of vision, possible perforation and loss eye.

Methods: This observational study was conducted in upgraded department of ophthalmology, BRD Medical College, Gorakhpur, India among 50 patients of suspected fungal keratitis.

Results:Fungal keratitis is more common in young males of rural area. Most of fungal corneal (>5 mm) with presence of hypopyon, pigmentation and satellite lesion as consistent features. KOH smear with impression smear technique was positive in 36 (72.0%), out of total 50 cases. KOH smear with conventional mechanical scrapings was positive in 35 (70.0%), out of total 50 cases. Sensitivity of impression smear technique as compared to conventional scraping was 100.0%. Sensitivity of mechanical scraping technique as compared to impression smear technique was 92.11%.             

Conclusions:Impression cytology is a recent diagnostic tool in fungal keratitis. It has various advantages over mechanical scraping like no expensive equipments like slit lamp and operation microscope required. Impression smear KOH mount is comparative to conventional mechanical corneal scraping KOH examination in making and tentative diagnosis of fungal keratitis and can be accurately replied upon for initiation antifungal therapy.

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Published

2016-12-09

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Section

Original Research Articles